James Gusella, PhD
Harvard Medical School
The Gusella lab investigates nervous system disease using molecular genetic strategies, beginning with human patients and proceeding through in vitro and modeling studies, with the ultimate goal of improving diagnosis, prevention, management and treatment.
In any given disorder, the research can usually be divided into four sequential stages:
- Determination of the chromosomal location of a gene defect, susceptibility gene or genetic modifier, usually based on linkage or association studies with polymorphic genetic markers or more recently by genome sequencing.
- Identification of the gene responsible for the phenotypic effect based upon its chromosomal location and/or sequence using a variety of genome analysis strategies.
- Characterization of the mechanism of action based upon analysis of the allelic versions of the culprit gene in humans and in appropriate in vitro or in vivo model systems, including cultured human cells, particularly iPS cells and their differentiated derivatives, genetically engineered mice, and lower organisms such as Drosophila and Dictyostelium.
- Exploration of the potential for rational therapies, including genetic therapies.
We are searching for susceptibility and modifier genes in neurodevelopmental disorders and in Huntington's disease.
As part of the Developmental Genome Anatomy Project, we also identify genes at breakpoints of balanced translocations associated with developmental abnormality.
We are also examining the mechanism of pathogenesis of genetic defects in autism, Huntington's disease, and neurofibromatosis, and pursuing assays to identify genetic and chemical modifiers, with the ultimate goal of contributing to effective rational therapies.