Date:
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Course Description
Clustered regularly interspaced short palindromic repeat (CRISPR) RNAs and their CRISPR-associated (Cas) proteins are an important part of adaptive immune systems in many prokaryotes. CRISPR-Cas systems function as RNA-directed endonucleases that can target nucleic acids in a sequence-specific manner and are now widely used as genome editing tools. In this course, we will provide lectures covering: an introduction to genome editing and cutting-edge improvements to CRISPR-Cas systems; a review of bioinformatics tools for guide RNA design and analysis of CRISPR-Cas data; and an overview of on-going and potential therapeutic applications of genome-editing nucleases. The course will also include a practical lab-based workshop for registered students in which participants will learn how to design guide RNAs and how to perform the broadly useful TIDE assay that enables quantification of nuclease-induced mutations in any cell or organism.
Nanocourse Director: J. Keith Joung
Curriculum Fellow: Emily Gleason (emily_gleason@hms.harvard.edu)
Lecturers
• J. Keith Joung, MD, PhD, Professor of Pathology, Harvard Medical School and Pathologist, Massachusetts General Hospital
• Luca Pinello, PhD, Instructor in Medicine, Massachusetts General Hospital Department of Pathology
• Morgan Maeder, PhD, Scientist, Editas Medicine
 | crispr_poster_compatibility_mode.pdf | 215 KB |