Flow Cytometry Cores

Since its beginning, HSCI established, launched, and helped operate several flow cytometry core facilities across HSCI institutions to ensure that key analytic resources would be available to our growing research community.

HSCI and BIDMC Flow Cytometry Core Facility

The Harvard Stem Cell Institute (HSCI) and Beth Israel Deaconess Medical Center (BIDMC) have made Flow Cytometry services available to HSCI Principal and Affiliated Faculty. Under the direction of John Tigges, Core Facility Manager, this service is managed by a full-time experienced technician, and HSCI Faculty will have priority and reduced pricing for the use of the BD FACSAria. The Laboratory is located in the BIDMC at 330 Brookline Avenue in Longwood. For more information and for the registration form, please contact John Tigges or download information about the core's equipment and capabilities.

HSCI and Children's Hospital Boston Flow Cytometry Core Facility

The Harvard Stem Cell Institute (HSCI) and Children's Hospital Boston have made Flow Cytometry services available to HSCI Principal and Affiliated Faculty. The lab provides flow cytometry services for analyzing and sorting of cells, as well as training and expert consultation for project/experiment planning. The lab is equipped with 2 FACSArias, an LSRII and a FACSCalibur and is directed and Managed by Ronald Mathieu with the help of Mahnaz Paktinat. For more information, visit the core's website.

HSCI and HMS Division of Immunology Flow Cytometry Facility

The Division of Immunology’s Flow Cytometry Facility provides flow cytometric analysis and cell sorting services to Division and Harvard Stem Cell Institute investigators. It also welcomes users from Harvard Medical School and affiliated institutions. The Facility is operationally connected to the Flow Cytometry Core at the Joslin Diabetes Center. For more information, please visit the core's website.

HSCI and HSCRB Flow Cytometry Core Facility

The Harvard Stem Cell Institute (HSCI) and Harvard Department of Stem Cell and Regenerative Biology (HSCRB) have made Flow Cytometry services available to HSCI/HSCRB Faculty. The HSCRB Facility provides a stable framework for multidisciplinary studies to investigators for cell analyzing and sorting abilities. The Core is under the direction of the Flow Cytometry Steering Committee members Douglas Melton, PhD, Jeffrey Macklis, MD, and Amy Wagers, PhD. The service is managed by two full-time experienced operators who provide assistance and training on three sorters and two analyzers. HSCI/HSCRB Faculty will have priority and reduced pricing for the use of all HSCRB instruments. The facility is located in the Sherman Fairchild Building on the main Harvard University Campus. For more information and for the registration form, please contact Joyce LaVecchio.

HSCI and Joslin Flow Cytometry Core Facility

The Harvard Stem Cell Institute (HSCI) and Joslin Diabetes Center have made Flow Cytometry services available to HSCI Faculty. The Joslin Diabetes Center provides a stable framework for multidisciplinary studies to investigators for cell analyzing and sorting abilities. The Core Director is Thomas Serwold, PhD, and Co-Director is Amy Wagers, PhD. The service is managed by two full-time experienced operators who provide assistance and training on three sorters and two analyzers. HSCI Faculty will have priority and reduced pricing for the use of all Joslin instruments. The facility is located in the Joslin Diabetes Center at One Joslin Place. For more information and for the registration form, please contact Joyce LaVecchio.

HSCI and MGH Flow Cytometry Core Facility

The Harvard Stem Cell Institute (HSCI) and Massachusetts General Hospital have made Flow Cytometry services available to HSCI Principal and Affiliated Faculty. Under the direction of Hanno Hock, this service is managed by a full-time experienced technician, and HSCI Faculty will have priority and reduced pricing for the use of the BD FACSAria. The facility is located at the Richard B. Simches Research Center on Cambridge Street, Boston. For more information please contact Laura B Prickett.

Longwood Medical Area CyTOF Mass Cytometer

A group of six institutions in Boston (Harvard Stem Cell Institute; Beth Israel Deaconess Medical Center; Brigham and Women's Hospital; Dana-Farber Cancer Institute; Harvard Medical School; and the Ragon Institute of MGH, MIT, and Harvard) joined forces to purchase and make available to the scientific community a CyTOF Mass Cytometer. CyTOF is an instrument that utilizes atomic mass spectrometry to allow simultaneous detection of up to 33 metal-based antibodies and two DNA intercalators for multi parameter high content single cell analysis without the limitations of marker emission overlap. The CyTOF is located in the Dana-Farber Cancer Institute FACS facility, Jimmy Fund Building Room 415. For more information please see the CyTOF website.