Job Type: Postdoctoral Fellow
Job Location: Center for Genomic Medicine, Massachusetts General Hospital
The Karmacharya lab is located in the Center for Genomic Medicine at Massachusetts General Hospital and part of the Harvard Program in Neuroscience and the Harvard Chemical Biology program. Our lab is interested in developing innovative stem cell-based models to interrogate the molecular and cellular underpinnings of neuropsychiatric disorders.
We are looking to recruit a highly motivated postdoctoral research fellow to join a multidisciplinary research group focused on studying the disease biology of neuropsychiatric disorders using human iPSC-derived brain organoids. The project will include single cell transcriptomic experiments, electrophysiological experiments in brain organoids with microelectrode arrays and CRISPR-mediated alterations to identify genes involved in modulating neural networks. The fellow will also work on developing methods for mechanistic studies of neurovascular and neuro-immune interactions using stem cell-based approaches. This is a unique opportunity for working in a highly collaborative research environment at the interface of chemistry, biology, and therapeutic development. The work will involve close collaborations with the Chemical Biology Program at the Broad Institute. The fellow will have simultaneous academic appointments at Harvard Medical School and Massachusetts General Hospital.
Ph.D. in neurobiology, stem cell biology, cell biology, or related field required. Must have 1-2 years experience in working with stem cells and have demonstrated expertise in the use of fluorescence microscopy, flow cytometry, cellular imaging/microscopy, and molecular biology (RT-PCR, cloning, immunoprecipitation, western blotting). Additional expertise in the use of CRISPR, RNA interference and viral vectors preferred. Must possess excellent computer skills and be able to perform quantitative analysis of large data sets. Advanced statistics or programming skills a plus. Excellent critical thinking skills and attention to detail needed. Must be able to use sound judgment to effectively solve problems, work independently, and handle a variety of tasks. Requires effective oral and written communication skills along with meticulous laboratory technique and recording skills.
Interested applicants should send cover letter, curriculum vitae, and the names, telephone numbers, and email addresses of three references to: Rakesh Karmacharya, MD, PhD firstname.lastname@example.org