Date:
Location:
Snacks will be provided. Please RSVP to nicole_paul@dfci.harvard.edu OR zeeshan.farooq@fluidigm.com
Speakers:
Bruce Bagwell, MD, PhD – President, Verity Software House
Greg Stelzer, PhD – Director-Mass Cytometry Market Development, Fluidigm
Abstract: The human hematopoietic cell population is a complex and dynamic system composed of morphologically and functionally diverse cell types. Mass cytometry combines the single cell analysis capability with the selectivity of mass spectrometry. Using antibodies conjugated to heavy metal isotopes, it enables quantitative analysis of high dimensional data on single cell level without confounding fluorescence overlap. With a significant increase in the number of simultaneous parameters measured, there is a need for computational tools to easily process and automate analysis of high-dimensional single-cell mass cytometry data.
In this seminar, we will introduce the Maxpar Human Immune Monitoring Panel Kit, a 29-marker panel designed to characterize immune cell types human peripheral blood. We will describe a bioanalytical system developed to characterize major immune cell subsets with data acquired on a Helios Mass Cytometer (CyTOF) and paired with an automated analysis software developed by Verity. Utilizing a unique combination of probability state modeling with Cen-se’ mapping, a t-SNE based algorithm optimized for mass cytometry data, a comprehensive visualization of high parameter data is generated. The automated analysis tool first cleans the file by automatically identifying and removing cell doublets, debris and dead cells. Then it identifies major myeloid and lymphoid lineage cell types, including cells at different stages of maturation.
Sponsored by:
LMA CyTOF Core
Dana-Farber Cancer Institute
450 Brookline Avenue
Boston, MA 02215
617-632-6561
LMA-cytof.dana-farber.org