Les E. Silberstein, MD
Harvard Medical School
A long-term objective of Dr. Silberstein’s research laboratory is to better define niche-induced signals and their intracellular pathways controlling HSPC development and function. In the bone marrow, hematopoietic stem and progenitor cells (HSPC) reside in close contact with stromal cells in distinct anatomical microenvironments, where they receive signals for lodgement/retention as well as growth and maturation. The approaches in the laboratory involve both human and murine (i.e. gene targeted) model systems along with imaging technology to better define the bone marrow cavity In-situ laser scanning cytometry is used to objectively quantitate the spatial distribution of hematopoietic stem and progenitor cells in bone marrow niches, while multi-photon intravital microscopy is used to define the dynamic behavior of HSPC and their adhesive interactions with specific niche cells. The research program is directly relevant to understanding the pathogenesis of normal and neoplastic hematopoietic disorders such as leukemia, myelodysplasia and immunodeficiency. Moreover, a better definition of the signaling pathways regulating HSPC cell fate could lead to new translational approaches to enhance ex vivo expansion and improve transplantation efficiency of clinically relevant HSPC populations.
Dr. Silberstein received his Baccalaureate and M.D. Degrees from the University of Leiden, the Netherlands, and had post-graduate training in Hematology/Oncology and Transfusion Medicine at Tufts-New England Medical Center in Boston, MA. He then joined the staff at The University of Pennsylvania, where he worked from 1983-2000. During this time Dr. Silberstein established an academic transfusion medicine division with the Department of Pathology and Laboratory Medicine. He also served as Director of the Blood Bank and Transfusion Medicine Section, and Associate Director, Bone Marrow Transplant Program.
Dr. Silberstein was then recruited to Harvard, where he is a Professor of Pathology at Harvard Medical School and Director of the Joint Program in Transfusion Medicine at Children's Hospital Boston, Brigham and Women's Hospital, and the Dana-Farber Cancer Institute. The Joint Program has three interrelated components: research, clinical and educational. An increasingly prominent activity within the Joint Program is the development of cell-based therapies. In addition, Dr. Silberstein is the Director of the Center for Human Cell Therapy (CHCT) at the Immune Disease Institute. The CHCT was established to provide an infrastructure to rapidly translate novel cell therapy protocols from the laboratory to the clinic. Anchored by its Translational Cell Therapy Laboratory and its Regulatory Core, the Center is designed to provide cell therapy resources for the Harvard community and its affiliated hospitals, from the technical level to the submission of Clinical Protocols and Investigational New Drug applications. He is also a Senior Investigator at the Immune Disease Institute and Head of the Harvard Stem Cell Institute’s Translational Research Program. Through both his leadership and research activities, he brings together Transfusion Medicine-related educational, research and clinical activities of the major Harvard Medical School affiliated institutions in Boston.